PD04-01 CORTISOL REGULATES ADRENALINE SYNTHESIS VIA PHENYLETHANOLAMINE N-METHYLTRANSFERASE IN SURGICAL SPECIMENS OF PHEOCHROMOCYTOMA

Abstract

You have accessJournal of UrologyCME1 May 2022PD04-01 CORTISOL REGULATES ADRENALINE SYNTHESIS VIA PHENYLETHANOLAMINE N-METHYLTRANSFERASE IN SURGICAL SPECIMENS OF PHEOCHROMOCYTOMA Kazuyuki Numakura, Yuya Sekine, Yumina Muto, Mizuki Kobayashi, Soki Kashima, Ryohei Yamamoto, Atsushi Koizumi, Taketoshi Nara, Mitsuru Saito, Shintaro Narita, and Tomonori Habuchi Kazuyuki NumakuraKazuyuki Numakura More articles by this author , Yuya SekineYuya Sekine More articles by this author , Yumina MutoYumina Muto More articles by this author , Mizuki KobayashiMizuki Kobayashi More articles by this author , Soki KashimaSoki Kashima More articles by this author , Ryohei YamamotoRyohei Yamamoto More articles by this author , Atsushi KoizumiAtsushi Koizumi More articles by this author , Taketoshi NaraTaketoshi Nara More articles by this author , Mitsuru SaitoMitsuru Saito More articles by this author , Shintaro NaritaShintaro Narita More articles by this author , and Tomonori HabuchiTomonori Habuchi More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002519.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Tyrosine hydroxylase (TYH), dopamine β-hydroxylase (DBH), and phenylethanolamine N-methyltransferase (PNMT) are key catecholamine synthetic enzymes (CSE) for biosynthesis from tyrosine and known for being coordinated by adrenal cortical hormones. However, little is known about the function and relationship of cortisol and CSEs for pheochromocytoma. In this study, we investigated an association between cortisol, CES, and catecholamines in pheochromocytoma patients treated by laparoscopic surgery (LA). METHODS: From September 1997 to January 2020, we performed LAs for 211 patients at the Akita University hospital. Of these, 39 patients were diagnosed with pheochromocytoma based on preoperative endocrinological evaluations and studied morning cortisol levels before surgery. Expressions of TYH, DBH, and PNMT were evaluated and classified positively by immunohistochemistry in the surgical specimens. RESULTS: The mean age of the patients was 59.0 years (range, 34–77 years; 14 men and 22 women). The mean tumor diameter was 4.3 cm (range, 1.8–8.5 cm). All laparoscopic surgeries were completed successfully. PNMT expression was very varied in individual patients (positivity: strong 17, intermediate 3, weak 7, and none 12) meanwhile, TYH and DBH were strongly expressed in all specimens. Morning cortisol value was associated with high plasma adrenaline level (correlation coefficient 0.376, p=0.034) (Figure 1). Additionally, patients with strong PNMT expression showed higher adrenaline level in both plasma (0.30 ng/ml vs 0.03 ng/ml, p=0.005) and urine (87.9 μg/day vs 21.1 μg/day, p=0.038) (Table 1). CONCLUSIONS: Cortisol secretion is positively related to adrenaline levels in pheochromocytoma patients. Strong PNMT expression is associated with a high level of adrenaline secretion. Thus, cortisol might regulate catecholamine synthesis via PNMT. Source of Funding: None © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e48 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information Kazuyuki Numakura More articles by this author Yuya Sekine More articles by this author Yumina Muto More articles by this author Mizuki Kobayashi More articles by this author Soki Kashima More articles by this author Ryohei Yamamoto More articles by this author Atsushi Koizumi More articles by this author Taketoshi Nara More articles by this author Mitsuru Saito More articles by this author Shintaro Narita More articles by this author Tomonori Habuchi More articles by this author Expand All Advertisement PDF DownloadLoading ...