PD03-05 ANTIBIOTICS AND A HIGH FAT/HIGH SUGAR DIET REDUCE MICROBIAL OXALATE METABOLISM IN A MOUSE MODEL

Abstract

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology I1 Apr 2018PD03-05 ANTIBIOTICS AND A HIGH FAT/HIGH SUGAR DIET REDUCE MICROBIAL OXALATE METABOLISM IN A MOUSE MODEL Aaron Miller, Teri Orr, Denise Dearing, and Manoj Monga Aaron MillerAaron Miller More articles by this author , Teri OrrTeri Orr More articles by this author , Denise DearingDenise Dearing More articles by this author , and Manoj MongaManoj Monga More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.271AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The incidence of urinary stone disease (USD) has increased 4-fold in the last fifty years. This emergent trend implicates systemic changes to public health as the primary driver behind the increase. The incidence of USD is associated with the gut microbiota both at the whole community level and at the level of specific functional species, such as the oxalate-degrading Oxalobacter formigenes. Thus, the objective of the current study was to test the hypothesis that antibiotics and a high fat/high sugar (HFHS) diet, both of which have increased over the last fifty years and has a significant impact on the gut microbiota, negatively impacts microbial oxalate metabolism in the gut. METHODS A high oxalate-degrading mouse model was developed by administering fecal transplants from the wild mammalian rodent Neotoma albigula to Swiss-Webster mice, which produces a microbiota that contains all of the bacteria necessary for persistent oxalate metabolism. One treatment group received its own feces as transplants as a negative control. Following transplants, animals were given either antibiotics, a HFHS diet, antibiotics in combination with a HFHS diet, or no treatment. Total oxalate metabolism and the composition of the gut microbiota was tracked over the ensuing two-week period. RESULTS Over the course of the diet trial, the Swiss-Webster mice with the native microbiota exhibited significantly lower oxalate degradation than animals receiving fecal transplants alone. Furthermore, there was a significant decline in oxalate degradation for animals receiving either the HFHS diet or the combination treatment. Interestingly, while animals receiving antibiotics initially saw a decline in oxalate degradation, the function had recovered by the end of the diet trial. CONCLUSIONS The results of our study indicate that diet had a greater effect on microbial oxalate metabolism than antibiotic use, implicating diet as a potential factor driving the increase in USD, through changes to the microbiota. This work has implications for the dietary management of USD and bacteriotherapies designed to inhibit the formation of stones. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e72 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Aaron Miller More articles by this author Teri Orr More articles by this author Denise Dearing More articles by this author Manoj Monga More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...