PD02-12 REVISITING THE ROLE OF INFLAMMATORY CYTOKINE-TARGETING APTAMERS IN CHRONIC PROSTATITIS

Abstract

You have accessJournal of UrologyCME1 Apr 2023PD02-12 REVISITING THE ROLE OF INFLAMMATORY CYTOKINE-TARGETING APTAMERS IN CHRONIC PROSTATITIS Tsun-Hsiang Cheng, Dong-Ru Ho, Hao-Chih Yang, Wai-Nga Chan, Jian-Hui Lin, and Pey-Jium Chang Tsun-Hsiang ChengTsun-Hsiang Cheng More articles by this author , Dong-Ru HoDong-Ru Ho More articles by this author , Hao-Chih YangHao-Chih Yang More articles by this author , Wai-Nga ChanWai-Nga Chan More articles by this author , Jian-Hui LinJian-Hui Lin More articles by this author , and Pey-Jium ChangPey-Jium Chang More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003219.12AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Chronic prostatitis is a non-infectious inflammatory disorder that plays an important role in the development lower urinary tract disease. Pro-inflammatory cytokines are key regulators of the inflammation process in prostate. IL-1β can directly attract innate inflammatory cells, cause tissue destruction, altered pain threshold, and initiate adaptive immunity. We hope to study the effectiveness of DNA aptamers to inhibit IL-1β mediated prostate inflammation in an experimental rat model of non-bacterial prostatitis (NBP). METHODS: In this study, we investigated the effects of inflammatory cytokine targeting aptamers (ICTA), a putative neutralizer of TNF-α and IL-1β activation, on local carrageenan-induced prostate inflammation in aged rats. The von Frey filament and tail-flick tests were performed to determine mechanical allodynia (a painful sensation caused by innocuous stimuli, e.g., light touch) and thermal hyperalgesia (a condition of altered perception of temperature), respectively, and prostate inflammation was examined 7 days after the administration of drugs. RESULTS: The levels of mononuclear cell infiltration, pro-inflammatory cytokine interleukin-1β, caspase 1(casp-1), and NALP 3 (NALP3) in the prostate of aged rats were increased 168 h after the NBP induction. Treatment with ICTA significantly attenuated carrageenan-induced allodynia, hyperalgesia, the increase in prostate glandular hyperplasia, and immune cell infiltration, and elevated levels of downstream cytokines including IL-18, TNF-α, and IL-33 in aged rats. Apoptosis markers, Bcl-2 and caspase-3, were found elevated. CONCLUSIONS: Our results showed that ICTA provides protection against local carrageenan exposure-induced enhanced pain sensitivity (allodynia and hyperalgesia), and that the protective effects may be associated with its ability to neutralize carrageenan-induced IL-1β activation, and result in apoptosis in the prostate of aged rats. Source of Funding: This research was funded by the Chang Gung Medical Foundation, Grant Number CMRPG6L0421-23, CORPG6M0011, CMRPG6L0211-12, CMRPG6M0161, NMRPG6M0071 © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e73 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tsun-Hsiang Cheng More articles by this author Dong-Ru Ho More articles by this author Hao-Chih Yang More articles by this author Wai-Nga Chan More articles by this author Jian-Hui Lin More articles by this author Pey-Jium Chang More articles by this author Expand All Advertisement PDF downloadLoading ...