PD01-06 DETECTION OF BACTERIA IN BLADDER MUCOSA OF ADULT WOMEN

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Interstitial Cystitis (PD01)1 Sep 2021PD01-06 DETECTION OF BACTERIA IN BLADDER MUCOSA OF ADULT WOMEN Stephen J. Walker, Robert Evans, Tyler Overholt, Mores Carine R, David J. Rademacher, Thomas Halverson, Roberto Limeira, Catherine A. Matthews, Gopal Badlani, Raymond Xu, Linda Brubaker, and Alan J. Wolfe Stephen J. WalkerStephen J. Walker More articles by this author , Robert EvansRobert Evans More articles by this author , Tyler OverholtTyler Overholt More articles by this author , Mores Carine RMores Carine R More articles by this author , David J. RademacherDavid J. Rademacher More articles by this author , Thomas HalversonThomas Halverson More articles by this author , Roberto LimeiraRoberto Limeira More articles by this author , Catherine A. MatthewsCatherine A. Matthews More articles by this author , Gopal BadlaniGopal Badlani More articles by this author , Raymond XuRaymond Xu More articles by this author , Linda BrubakerLinda Brubaker More articles by this author , and Alan J. WolfeAlan J. Wolfe More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001965.06AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic urological condition diagnosed in nearly 8 million women in the US. Whether the urinary microbiota plays an etiologic role remains controversial. Most studies have assessed the microbiota of IC/BPS patients with voided or catheterized urine as a proxy for bladder urothelium and although urine is a convenience sample, it may not be a true reflection of the bladder microbiota. The use of bladder biopsy tissue may provide a more accurate, and thus more clinically relevant, picture of the bladder microbiota. METHODS: Bladder biopsy tissues were obtained from: (a) 30 women with IC/BPS (18-80 y/o) via cystoscopically guided cold-cup biopsy following therapeutic bladder hydrodistension, and (b) 10 non-IC/BPS women undergoing pelvic organ prolapse repair. To detect bacteria, technical duplicates of each RNAlater-preserved biopsy were subjected to 16S rRNA gene sequencing. To visualize bacteria, paraformaldehyde-fixed, paraffin-embedded biopsies were subjected to combined fluorescence in situ hybridization (FISH) and fluorescence immunohistochemistry (IHC) using confocal microscopy. RESULTS: Bacteria were detected in at least one technical replicate of every biopsy (Fig 1). The most abundant genus was Staphylococcus (light blue), followed by Lactobacillus (dark blue); Escherichia (gold) was common but not abundant. Combined FISH/IHC reproducibly detected perinuclear clusters of 16S RNA in epithelial cells of human bladder biopsy tissue (Fig 2). There was no significant difference between IC/BPS patients and controls (p>0.05). CONCLUSIONS: In this study we were able to detect and visualize bacteria associated with the bladder urothelium in 40 women; we conclude that urothelial and urinary microbiota are similar but not identical (e.g., Lactobacillus is more abundant in urine; Staphylococcus is more abundant in the urothelium). We did not find evidence that urothelial microbiota differs between women with IC/BPS and non-IC/BPS controls. Much larger studies are indicated. Source of Funding: 1R01DK124599-01 (Walker, PI); NCATS UL1TR001420 (Walker, PI); R01DK104718 (Wolfe & Brubaker, multi-PI) © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e32-e33 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Stephen J. Walker More articles by this author Robert Evans More articles by this author Tyler Overholt More articles by this author Mores Carine R More articles by this author David J. Rademacher More articles by this author Thomas Halverson More articles by this author Roberto Limeira More articles by this author Catherine A. Matthews More articles by this author Gopal Badlani More articles by this author Raymond Xu More articles by this author Linda Brubaker More articles by this author Alan J. Wolfe More articles by this author Expand All Advertisement Loading ...