Abstract
Powdery mildew (PM) is a fungal disease occurring in both field and greenhouse conditions worldwide. It infects many plant species and reduces both the productivity and quality of crops. Melon (Cucumis melo L.) is an economically important crop. In order to develop a molecular marker that can be used more conveniently in the development of PM-resistant melon using MR-1 melon resources, the previously reported cleaved amplified polymorphic sequence (CAPS) marker was improved with a length polymorphism PCR marker. Two cleaved CAPS markers—BSA12-LI3ECORI and BSA12-LI4HINFI—associated with BPm12.1, a major quantitative trait locus (QTL) corresponding to the PM resistance of MR-1, have been reported. In this study, we found that in the BSA12-LI3ECORI CAPS marker specifically, a 41 bp deletion was present in the PCR DNA region of the MR-1 melon genome. A new marker capable of distinguishing polymerase chain reaction (PCR) length polymorphism was produced using insertion-deletion (InDel) information in this region. This PCR-based InDel marker distinguished the genotypes of PM-resistant MR-1, PM-susceptible Top Mark, and their F1 progeny. These results suggest that this InDel marker could be used to develop PM-resistant melon varieties based on MR-1.
Highlights
Melon (Cucumis melo L., Cucurbitaceae) is an economically important crop with a worldwide production of more than 29 million tons per year [1]
We developed a polymerase chain reaction (PCR)-based InDel marker that can discriminate MR-1 resistance more efficiently by using information from BSA12-LI3ECOR1 and BSA12-LI4HIFI, which are cleaved amplified polymorphic sequence (CAPS) markers associated with BPm12.1 quantitative trait loci (QTL), associated with MR-1 powdery mildew (PM) resistance
Since it is close to the BPm12.1 powdery mildew resistance QTL at 0.2 cM [26], it is expected that the BSA12-LI3ECORI CAPS marker itself can be used as a BPm12.1 resistance marker
Summary
Melon (Cucumis melo L., Cucurbitaceae) is an economically important crop with a worldwide production of more than 29 million tons per year [1]. Powdery mildew (PM) reduces the productivity of all Cucurbitaceae crops worldwide. Molecular markers used for breeding are primarily aimed at a single gene associated with the target trait [10,11,12,13,14]. Genetic linkage analysis is mainly applied using quantitative trait loci (QTL) derived from between two parents with a contrasting phenotype. With this method, DNA markers for phenotypic data with characteristics of interest are identified based on variations in DNA sequences in segregation generations. The targeted gene region is introduced into an agriculturally superior elite line using crosses, and used as an association marker until the target trait gene is stably inherited in the elite plant [15]
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