Abstract
Oxidative stress plays an important role in the induction of cell death and is associated with various pathologic disorders; therefore, the search for natural products that attenuate the effects produced by oxidant agents is greatly increased. Here, the protective effects of native lectin from Cratylia mollis seeds (pCramoll) and recombinant Cramoll 1 (rCramoll) against H2O2-induced oxidative stress in Vero cells were evaluated. Both lectins significantly attenuated the H2O2-induced cytotoxicity in a concentration-dependent way. The maximum protective effects were 96.85 ± 15.59% (rCramoll) and 59.48 ± 23.44% (pCramoll). The Live/Dead analysis showed a reduction in the percentage of dead cells from 65.04 ± 3.29% (H2O2) to 39.77 ± 2.93% (pCramoll) and 13.90 ± 9.01% (rCramoll). The deleterious effects of H2O2 on cell proliferation were reduced to 10.83% (pCramoll) and 24.17% (rCramoll). Lectins treatment attenuated the excessive superoxide production, the collapse of the mitochondrial membrane potential, and the lysosomal and DNA damage in H2O2-treated cells. In conclusion, our results suggest that pCramoll and rCramoll blocked H2O2-induced cytotoxicity through decreasing reactive oxygen species, restoring the mitochondrial potential, preventing the lysosomal damage and DNA fragmentation, and thus promoting cell survival and proliferation.
Highlights
Oxidative stress is characterized by an imbalance in the redox status of the cell and has been implicated in a range of age-associated and neurodegenerative diseases, such as aging, cancer, diabetes, Alzheimer’s disease, and Parkinson’s disease [1]
We found that the cytoprotective effects of these lectins are mediated by the decrease of superoxide species production that prevent the mitochondrial and lysosomal dysfunctions and the DNA damage
The cytoprotective effects of lectins against H2O2-induced cell death were evaluated by MTT assay, which measures the loss of metabolic activity of cells and it is an early indicator for cell death
Summary
Oxidative stress is characterized by an imbalance in the redox status of the cell and has been implicated in a range of age-associated and neurodegenerative diseases, such as aging, cancer, diabetes, Alzheimer’s disease, and Parkinson’s disease [1]. Oxidative Medicine and Cellular Longevity of normal metabolic processes They include superoxide anions (O2−), hydroxyl radicals (OH∙) and hydrogen peroxide (H2O2; nonradical derivative of oxygen). ROS may damage relevant classes of biological macromolecules in the cells through direct oxidation of lipids, proteins, and nucleic acids, thereby disrupting cellular function and integrity, which leads to cell death [1, 3]. Cramoll 1,4 (preparation containing isolectins 1 and 4; pCramoll) is isolated in a similar way to concanavalin A (Con A), a well-known lectin from Canavalia ensiformis seeds [8]. This preparation has shown interesting biological activities such as immunomodulatory, antitumoral, antiparasitic, and healing agent [9]. We found that the cytoprotective effects of these lectins are mediated by the decrease of superoxide species production that prevent the mitochondrial and lysosomal dysfunctions and the DNA damage
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
