Abstract
Real-time PCR assay (the TaqMan probe method) was evaluated for the detection of DNA of genus Erysipelothrix directly from chronic arthritic fluids of swine with erysipelas. By using a QIAamp DNA Mini Kit (Qiagen) for DNA extraction, the time frame was less than 4hrs. With this method, in most cases the number ofErysipelothrixorganisms in the synovial fluids was estimated to be less than 102 cfu per milliliter. The combination of the test results of synovial fluids by enrichment cultivation, real-time PCR and antibody assay by Surface protective antigen (SpaA)-ELISA may give information that is important to understanding the condition of the disease.
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