Abstract
Molecular biology techniques genetic improvement by facilitating identification, mapping and analysis of polymorphism of genes by encoding proteins that act on metabolic pathways involved in economically interesting traits. This use of genetic markers can aid identification of those animals with the highest breeding values in sheep. On the basis of sheep genome mapping, information was examined on the ovine IGF1 and PIT1 genes as a possible genetic marker for growth traits in sheep. The current study was designed to estimate the frequencies of putative IGF-1 and PIT-1 genes SNPs and investigate associations with calculated EBVs of growth traits in Makooei sheep. PCR-SSCP analysis of the exon1 of IGF-I gene and include a part of intron2, exon3 and a part of intron3 and PIT-1 gene revealed the following banding patterns; three (AA, AG, GG) and four AA (p1), AB (p2), CC (p3), CD (p4), banding patterns respectively. Results from this study demonstrated higher performance of AA animals in BW and GBW, and AG animal in WW and W6 that may be related to the role of IGF-1 at the pre-puberty and puberty stages. Also higher performance of p3 animals in W9, YW and GSN, and p1 animal in GNY may be related to the PIT-1 role in post-puberty.
Highlights
The Makooei sheep are one of the Iranian fat-tailed, mediumsize breeds
Estimates of variance and covariance components were based on animal models using the restricted maximum likelihood (REML) approach in a derivate-free (DF) algorithm [14]
The current study was designed to estimate the effect of Single Strand Confirmation Polymorphism (SSCP) patterns on weight estimated matenal breeding values for body weights prior to 6-months of growth traits
Summary
The Makooei sheep are one of the Iranian fat-tailed, mediumsize breeds. They are distributed in the mountainous areas of the country, especially in West Azerbaijan province. They are found in Turkey and called White Karaman. The GH gene pathway contains various interdependent genes, such as GH, insulinlike growth factor (IGF1), pituitary specific transcription factor (PIT1), growth hormone releasing hormone (GHRH), somatostatin growth hormone releasing hormone receptor (GHRHR), growth hormone receptor (GHR), and others [5]. GH, GHR, insulin-like growth factor I (IGF-I), leptin (LEP), caprine-pituitary-specific transcription factor-1 (POU1F1), caprine myostatin (MSTN), and bone
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