Abstract
Bacterial shoot blight is a disease that has the potential to have a significant impact on tea cultivation in Shizuoka prefecture. To enable the easy and rapid detection of the causal agent of this disease, a direct polymerase chain reaction (PCR) method was developed using samples from tea leaves. A piece of tea leaf that included a water-soaked lesion was ground down in sterile water. After the debris had settled, the suspension was boiled and then used as a PCR template. When the undiluted supernatant was used as the template, marked PCR interference occurred. In contrast, a clear target band was detected when a 10-fold dilution of the supernatant was used as the template. This method was shown to be an easy and rapid way identifying the causative pathogen of bacterial shoot blight on tea plants.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Annual Report of The Kansai Plant Protection Society
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
