Abstract
We utilized the reverse transcription-polymerase chain reaction (RT-PCR) to detect the presence of basic fibroblast growth factor (bFGF) messenger RNA in rat brain, ovary and kidney. The nucleotide sequence of the RT-PCR product revealed a novel 3′ untranslated (UT) sequence in the rat basic FGF mRNA. In this sequence, as in the 3′ UT regions of many growth regulatory genes, there is a high degree of conservation of A + T rich motifs which have been shown to play a major role in mRNA stability.
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