Abstract
We designed two novel primer sets based on the sequence of the hrpZ gene to detect Pseudomonas syringae pv. syringae, the causal agent of bacterial black node in barley and wheat. A conventional PCR using these primers amplified only P. syringae group III strains. The result of a nested PCR with the novel primers showed that P. syringae pv. syringae was detectible even when only one culturable cell was used as the PCR template. This highly sensitive PCR detected P. syringae in diseased parts of wheat and barley plants and in asymptomatic plants.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.