Abstract
Olive oil adulteration with other cheap oil species has long been upsetting the industry, consumer, and authority around the world. In this article, we first reported the application of PCR-CE-SSCP method to detect other oil species in olive oil. The method was proved to be fast, accurate, reproducible, and interpretation easy. SSCP profiles of 7 oil species were all characterized by single amplicon peak which differentiate olive and the other plants distinctly. The method detection limit was less than 10% of other plant DNA blended with olive DNA. The practical limit for oil was 30–50% refined soybean oil in olive oil.
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