Abstract
Mercury resistant Pseudomonas strains were isolated from polluted water, rhizospheric soil and root nodules of different plant species. Selected bacterial isolates showed potential to produce florescence under UV light and to tolerate heavy metal stress of 10-20 µg/ml HgCl2. Biochemical characterization revealed all selected bacterial isolates belong to genus Pseudomonas. A total of 27 bacterial isolates were evaluated for bioremediation of mercury due to their ability to produce H2S and fix nitrogen. Genomic DNA of 12 Pseudomonas strains was isolated and their genetic diversity was evaluated using SSR (GACA)4 microsatellite finger printing. On the basis of banding pattern of PCR amplified products, a phylogenetic tree was constructed using Minitab 13 software which showed 71%, 46% and 44% homology of SZ-30, SZ-6 and SZ-8 with strains of cluster 1 and SZ-16 respectively. Results exhibited significant diversity among selected Pseudomonas strains.
Highlights
The microbial diversity is the current frontier in biology which still needs much exploration
Plant microbe interaction is very beneficial and detrimental process for proper maintenance of soil texture, plants and microbes respectively [4]. Such free living bacteria benefit to plants by (1) production of phytohormones like auxins and derivatives of IAA (2) N2 fixation by asymbiotic processes (3) antagonistic activity against pathogenic bacteria of plants
For the isolation of N2 fixing mercuryresistant fluorescent bacteria, 100 μl of each bacterial culture was spread on NFM agar plates and incubated for 2-3 days at 37 oC
Summary
The microbial diversity is the current frontier in biology which still needs much exploration. Screening of mercury-resistant fluorescent bacteria by well plate method Cellular and biochemical characterization of isolated strains
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