PCR-based Differentiation Of Zoonotic Fascioliasis

Abstract

Out of 100 samples from slaughtered cattle of different ages were examined at Kalyobia province slaughterhouses Tukh center from October 2010 to October, 2011 in order to identify associated risk factors, 8% gave positive results (6 Fasciola hepatica and 2 Fasciola gigantica). Out of 100 stool samples examined from men occupationally contacts with cattle, 2% were positives to fasciola hepatica only. The histopathological examination of the cattle liver infested with fasciola species showed newly formed bile ductules with inflammatory cells infiltration and fibrosis associated with hyperplasia in the lining epithelium with polyps formation as well as the portal area showed severe fibrosis with inflammatory cells infiltration in both fasciola species lesions. Regarding PCR technique, the using of Eael restriction endonuclease enzyme as a genetic marker for F.hepatica is greatly effective when the enzyme uniquely fragmented the SrRNA gene into two bands. That means PCR could help to evaluate the relative zoonotic potential of Fasciola hepatica and Fasciola gigantica, especially in those regions, which have huge increases in the incidence of human fascioliasis in recent years.