Abstract
On Earth there are several organisms that share close relationship with humankind and Saccharomyces cerevisiae is one of them. Saccharomyces cerevisiae is also known as the brewer´s or baker´s yeast and has been used for fermentation and production of bread and alcoholic beverages since long time. The purpose of this study was to evaluate the fermentation capability of Saccharomyces cerevisiae local isolates. For this purpose 100 fruit samples (mango, sugarcane, orange, honey melon, grapes) were collected from local fruit markets and subjected to identification by standard microbiology tests and advance molecular techniques like Polymerase reaction test (PCR). The results revealed the typical growth, shape and color of S. cerevisiae on YPD media. The growth culture subjected to microscopy showed the budding characteristic of S. cerevisiae. The initial identified strains by virtue of YPD selective media and microscopy were subjected to PCR. The PCR results revealed the presence of S. cerevisiae in 28 samples among all analyzed samples. Importantly, the presence of S. cerevisiae was detected 30% in oranges, in mango 27.77%, in sugarcane 35% while in honey melon and grapes it was 13.63%, 31.81% respectively.
 This study concludes that S. cerevisiae was more abundant in sugarcane and grapes analyzed samples. The collected strains of present study may be further used in future studies like ethanol production from raw materials and also used as reference strain when working with samples of unknown nature
Highlights
In recent decades Pakistan has faced the severe energy crisis which necessitates studying and implementing the alternatives sources of energy [1]. In this regard ethanol fermentation serves the best alternative source as raw material required is available in abundance like sugarcane, orange peel other rotten fruits like grapes, mango and melons [2]
S. cerevisiae is mainly used as fermenting yeast in ethanol production when using the sugar cane or other food/fruits by products as raw material [12, 13]
In this study Saccharomyces cerevisiae was isolated from five different rotten fruits (Sugarcane,Oranges, Grapes, Honey melon and Mango)
Summary
In recent decades Pakistan has faced the severe energy crisis which necessitates studying and implementing the alternatives sources of energy [1]. In this regard ethanol fermentation serves the best alternative source as raw material required is available in abundance like sugarcane, orange peel other rotten fruits like grapes, mango and melons [2]. To produce ethanol from raw materials yeast fermentation has been recognized as the most efficient and economical in the biotechnology industry. Almost 80% of the ethanol produced is carried out by help of Saccharomyces cerevisiae which ferments the sugar sources.
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