Abstract
ZSUZSANNA KONCZ, Z. NAAR, A. KISS and A. SZECSI Laboratory of Molecular Biology, Egerfood Regional Research Centre, Eszterhazy Karoly College, H-3300 Eger, Hungary Plant Protection Institute, Hungarian Academy of Science, P.O. Box 102, H-1525 Budapest, Hungary Enniatins (Ens) are cyclic hexadepsipeptides consisting of alternating amino acid residues with antimicrobial, insecticidal and phytotoxic activities [1]. Fusarium avenaceum, F. poae, F. sporotrichioides and F. tricinctum are the main source of Ens [2, 3]. The aim of the my study was application of different PCR primer pairs based on esyn1 gene encoding multifunctional enzyme enniatin synthetase for the detection of potential enniatin-producing Fusarium species originated from Hungarian wheat grain. Primers used in multiplex PCR amplified products from the Fusarium avenaceum (18 isolates), F. poae (13 isolates), F. sporotrichioides (3 isolates) [4]. The current study has demonstrated that all Hungarian isolates were positive for capable of producing Ens, may have an underestimated toxic potential. This is a first data of enniatin-producing potential of Hungarian Fusarium species. 1. Firakova, S., Proska, B., Sturdikova, M.: Biosynthesis and biological activity of enniatins. Pharmazie 62, 563–568 (2007). 2. Nicholson, P., Simpson, D. R., Wilson, A. H., Chandler, E., Thomsett, M.: Detection and differentiation of trichotheceneand enniatin-producing Fusarium species on small-grain cereals. European J Plant Pathol 110, 503–514 (2004). 3. Ivanova, L., Skjerve, E., Eriksen, G. S., Uhlig, S.: Cytotoxicity of enniatins A, A1, B, B1, B2 and B3 from Fusarium avenaceum. Toxicon 47, 868–876 (2006). 4. Kulik, T., Pszczotkowska, A., Fordonski, G., Olszewski, J.: PCR approach based on the esyn1 gene for the detection of potential enniatin-producing Fusarium species. Int J Food Microbiol 116, 319–324 (2007). 1217-8950/$20.00 © 2008 Akademiai Kiado, Budapest Acta Microbiologica et Immunologica Hungarica, 55 (3), p. 365 (2008) DOI: 10.1556/AMicr.55.2008.3.8 * Abstract of the Fourth Hungarian Conference of Mycology, May 29–31, 2008, Debrecen, Hungary
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