Abstract
Abstract A modified extraction procedure for wheat triticin allowed a better resolution of the triticin bands on SDS-PAGE. The modified protocol was used for the verification of Chinese Spring ditelocentric stocks before DNA extraction for PCR amplification of triticin gene segments. Three pairs of PCR primers were designed for specific amplification of two overlapping segments of the triticin gene including its hypervariable region. Consistent amplification was achieved with only one pair of primers for which the PCR conditions were further optimised. The bulk of the amplified product was due to genes on the short arm of chromosome 1D. Hence, under the given conditions there was a preferential amplification of the Tri-D1 gene. These primers will be useful in screening for natural variation in the triticin HVR segment in wild wheat collections.
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