PCR Amplification of a novel Transcription Initiation Factor gene ( TI‐1) in the Smooth Muscle Cells of Gastro Intestinal Tract of Rabbit and Phylogenetic sequence analysis

Abstract

Initiation of Translation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. We have identified a novel transcription initiation factor (TI-1) in the smooth muscle cells of Gastrointestinal tract of Rabbit using with a forward primer and a reverse primers. Bioinformatics analysis of the PCR product revealed that the sequence is closely related with Homo sapiens eukaryotic translation initiation factor 4 gamma 2 (IEF4g2) splice variant 2 mRNA with 97% homology. Phylogentic analysis revealed this region is closely related with human chromosome 11 location 11p15 with a score of 351 and E value of 8e-95 and the sequence is more correlated to the group of primates with a sequence difference of only 0.75 in the course of evolution by Neighbour joining tree method with a boot strap value of 10000. Structurally observed that this gene closely related to the X-ray strucutre of phylogenetically conserved middle region of human eukaryotic initiation factor (eIF) 4GII revealing a crescent-shaped domain consisting of ten alpha helices arranged as five HEAT repeats. Together with the ATP-dependent RNA helicase eIF4A, this HEAT domain is resposponsible for 48S ribosomal complex formation with a picornaviral RNA internal ribosome entry site.