Abstract
Increasing issues of pathogen drug resistance and spreading pose a serious threat to the ability to treat common infectious diseases, which encourages people to explore effective technology to meet the challenge. Photodynamic antibacterial inactivation (aPDI) is being explored for inactivating pathogens, which could be used as a novel approach to prevent this threat. Here, porphyrin-embedded MOF material (PCN-224) with photodynamic effect was synthesized, then the PCN-224 nanoparticles (NPs) were embedded into PAN nanofibers with an electrospinning process (PAN-PCN nanofiber membrane). On the one hand, polyacrylonitrile (PAN) nanofibers help to improve the stability of PCN-224 NPs, which could avoid their leakage. On the other, the PAN nanofibers are used as a support material to load bactericidal PCN-224 NPs, realizing recycling after bacterial elimination. An antibacterial photodynamic inactivation (aPDI) study demonstrated that the PAN-PCN 0.6% nanofiber membrane processed 3.00 log unit elimination towards a E. coli bacterial strain and 4.70 log unit towards a S. aureus strain under illumination. A mechanism study revealed that this efficient bacterial elimination was due to singlet oxygen (1O2). Although the materials are highly phototoxic, an MTT assay showed that the as fabricated nanofiber membranes had good biocompatibility in the dark, and the cell survival rates were all above 85%. Taken together, this work provided an application prospect of nanofibers with an aPDI effect to deal with the issues of pathogen drug resistance and spreading.
Highlights
Since we entered the 21st century, pathogens such as virus, fungi, bacteria and even parasites have become increasingly aggressive to human beings, and infectious diseases have become more violent in recent decades
The reactive oxygen species (ROS) detection study indicated that light-driven 1 O2 is responsible for bacterial inactivation, the antibacterial photodynamic inactivation (aPDI) behavior of polyacrylonitrile nanofiber membrane with high aPDI efficiency (PAN-Porous Coordination Network (PCN)) nanofiber membrane functions through a Type II mechanism
The aPDI study employing the electrospun PAN-PCN nanofibers was performed with model bacterial strains of E. coli and S. aureus, and the survival rates of bacterial cells are The shown
Summary
Since we entered the 21st century, pathogens such as virus, fungi, bacteria and even parasites have become increasingly aggressive to human beings, and infectious diseases have become more violent in recent decades. Pathogen inactivation by ROS-induced damage has the following important advantages [6]: i) antibiotic-resistant pathogens exhibit virtually equal levels of inactivation as their antibiotic-susceptible counterparts; ii) because of the non-specific damage caused by ROS, development of microbial resistance to aPDI is highly improbable. The ROS detection study indicated that light-driven 1 O2 is responsible for bacterial inactivation, the aPDI behavior of PAN-PCN nanofiber membrane functions through a Type II mechanism. This research provides a facile approach to embed PCN-224 NPs into PAN nanofibers, in order to develop a novel nanomaterial with aPDI effect to deal with the issues of pathogen spread and infectious disease
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