PCL Based CIP-Loaded Double-Layer Films Deposited by Low-Electron Beam Dispersion Method and its Antibacterial Properties

Qin Xu,Beibei Li,A.A Rogachev,A.V Rogachev,Zhengwei Xu,M.A Yarmolenko,Xiaohong Jiang

doi:10.18321/ectj997

Abstract

In this paper, low-electron beam dispersion (EBD) method is used to prepare a kind of double-layer films on different substrates. The bottom layer is a mechanically stirred mixture of the degradable polycaprolactone (PCL) and ciprofloxacin (CIP), and the top layer is polyurethane (PU) film. The molecular structure, chemical composition and morphology of the double-layer films were investigated by FTIR, XPS and SEM. The results showed that the surfaces of the double-layer films are uniform and the thicknesses can reach micron level. In addition, the two layers are well bonded. Then the films were sliced and immersed in PBS solution, and the time-dependent variable was used to analyze the kinetic slow-release behavior of CIP in the double-layer films by agar diffusion antibacterial experiments. It can be seen that sustained release time of CIP in the double-layer films can be up to 7 days, which is due to fact that the upper PU film working as a sealing layer helps to realize the drug slow-release. Based on the above research, the comprehensive performance of the films with the composition of PCL:CIP/PU=1:1/1 is the best.

Highlights

Nowadays, most artificial implants used in hospitals are facing various problems such as inflammation, rejection, allergic reactions and biofilms produced from varying degrees of pathogen adhesion [1]
In Fig. 1/3, after mechanical mixing and electron beam dispersion (EBD) deposition, the IR spectrum of the single-layer film (PCL:CIP=1:1, mass ratio) is characterized by the presence of a broad absorption band at 1100‒1300 cm-1 caused by the stretching vibration of C-O-C bond, which exists in both PCL and CIP [16]
From FTIR and XPS analysis, it is confirmed that PCL:CIP single-layer film and PCL:CIP/PU double-layer films were successfully prepared on substrates by EBD method

Summary

Introduction

Most artificial implants used in hospitals are facing various problems such as inflammation, rejection, allergic reactions and biofilms produced from varying degrees of pathogen adhesion [1]. With the purpose of ensuring that implant materials like hip and knee joints can continuously and stably work in the human environment, it is essential to achieve biofilm inhibition and reduce bacterial infections. We are committed to depositing biodegradable drug-loaded polymer films on titanium (Ti) substrates to regulate drug delivery and endow long-term antibacterial properties [2,3]. Poly-ε-caprolactone (PCL) is a biodegradable and biocompatible semi-crystalline polymer with a low glass transition temperature (-60 °C) [6]. When in the human’s body, the cells can grow normally on their base frame and can be degraded into CO2 and H2O. PU is a nontoxic polymer with prominent biocompatibility, biodegradation, antibacterial ability and mechanical properties including wear resistance and easy processing, which possesses promising application prospect in a variety of biomedical fields especially controlled drug release [10]

Methods

Results

Conclusion