Abstract
Automatic counting of yeast cells in baker's yeast culture was tried using a conventional light microscope equipped with a pc camera. Relatively good binary image was obtained by using white LED as microscope light source, but uneven brightness distribution in original image hindered counting accuracy. A block binarization method using local thresholds proportional to local brightnesses was used to get improved binary images. The brightnesses of the blocks were expressed as the value component in HSV color model. Good quality binary images were obtained by binarization on <TEX>$8{\times}6$</TEX> blocks of original images and connected-component labelling of the binarized images produced reliable counting results in the concentration range <TEX>$1.4{\times}10^5/mL{\sim}1.4{\times}10^7\;cells/mL$</TEX>.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
