PBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination

S Boukthir,P Gaudu,A Faili,S Kayal

doi:10.1016/j.heliyon.2023.e16720

PBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination

S Boukthir, P Gaudu + Show 2 more

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https://doi.org/10.1016/j.heliyon.2023.e16720

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Journal: Heliyon	Publication Date: May 29, 2023
Citations: 1	License type: cc-by-nc-nd

Affiliation: Inserm, Centre Hospitalier Universitaire de Rennes, Micalis Institute, Agro ParisTech, National Research Institute for Agriculture, Food and Environment, University of Paris-Saclay, Institut Polytechnique de Paris, Oncogenesis Stress Signaling, University of Rennes

#Group A Streptococcus #Mutagenesis Strategy + Show 8 more

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Abstract

Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS emm3 genotype.

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