Patterns of unscheduled DNA synthesis in mouse embryo cells associated with in vitro aging and with spontaneous transformation to a continuous cell line

Abstract

Monolayer cell cultures derived from B/C mouse embryos were examined for the ability to repair ultraviolet light-induced DNA damage (50–250 erg/mm 2) during in vitro aging and subsequent alteration to a continuous cell line. Excision repair was measured by incubating the cultures with [ 3H]TdR and measuring DNA specific activity, and by performing quantitative autoradiography. DNA repair capacity declined during in vitro aging, and the level of repair correlated with the fraction of cells which retained the capacity to undergo scheduled DNA synthesis. This result indicates that mouse cells aged in vitro undergo a decline in their ability to repair UV-induced DNA damage comparable to that seen in cultured human fibroblasts. In cultures which spontaneously altered into continuously proliferating cell lines, DNA repair capacity increased to high levels, as did the fraction of cells capable of initiating scheduled DNA synthesis.