Patterns of microtubule organization in two polyhedral cell types in the gametophyte of the liverwort Marchantia paleacea Bert.

Abstract

The typical scale cells (TSCs) of Marchantia paleacea Bert, contain a well-developed cortical microtubule (Mt) cytoskeleton, particularly below the anticlinal walls and also display complete but broad preprophase-prophase Mt bands (PMBs). In contrast, the cortical cytoskeleton of the inner thallus cells (ITCs) is less developed than that of TSCs and the PMBs are incomplete. The latter consist of one to four separate Mt bundles which lie on the cytokinetic plane, but do not form a complete Mt ring. In both cell types PMB formation precedes or keeps pace with the activation of the polar Mt-organizing centres (MTOCs) and nuclear shaping. The Mts in the PMBs are more numerous and densely packed at the cell edges than on the cell face. The polar MTOCs persist up to late prophase-prometaphase. Afterwards, the spindle Mts are focused on several minipoles, where endoplasmic reticulum is localized. In postcytokinetic cells the cortical Mts first reappear on the daughter wall surface. Our findings suggest that: (a) The formation of complete or incomplete PMBs in TSCs and ITCs of M. paleacea is related to differences in the development of the interphase cortical Mt arrays, (b) The cell edges are able to form or at least arrange the Mts of the PMB. (c) Tight mature PMBs like those found in flowering plant cells are not formed in the cells examined in the present study. (d) The final orientation of the cell plate is controlled by the PMB cortical zone. (e) The cytoplasm abutting on the postcytokinetic daughter wall has the ability to assemble cortical Mts.