Pattern of selected calcium-binding proteins in the vestibular nuclear complex of two rodent species

Abstract

The distribution of immunoreactivity to calbindin, calretinin, and parvalbumin in the vestibular nuclear complex and the adjacent nucleus prepositus hypoglossi was studied in rats and gerbils. The distribution of stained fibers was the same for both rodent species. All three calcium-binding proteins were present in vestibular afferents. However, none of the three proteins were present in all afferent fibers. Many fibers were labeled in the vestibular nerve and in fascicles of the descending vestibular nucleus, as well as ascending fibers in the superior vestibular nucleus and fibers directed to the medial vestibular nucleus. Labeled terminals were present in the medial vestibular nucleus, especially along the ventricular border, in the neuropil of the superior vestibular nucleus, and scattered in the descending and ventral portions of the lateral vestibular nucleus. Calbindin- and parvalbumin-positive terminals, but not calretinin-positive terminals, were present in the neuropil of the dorsal lateral vestibular nucleus, especially surrounding the large neuronal somas. Some of these terminals are presumably from cerebellar Purkinje cells, which were also labeled by both antibodies. The pattern of parvalbumin immunoreactivity was slightly different from that of calbindin, indicating that parvalbumin might be contained in additional fibers. Some neurons in the vestibular nuclear complex were labeled with antibodies to calretinin, but few cells were stained with either calbindin or parvalbumin antibodies. The largest group of calretinin-positive cells was a cluster of small- to medium-sized neurons located in a densely stained mesh of dendrites and terminals in the medial vestibular nucleus, adjacent to the ventricular border.