Pattern of nonadrenergic, noncholinergic responses during short- or long-lasting electrical stimulation in guinea-pig ileum

Abstract

1. 1. The pattern of responses of longitudinally oriented guinea pig ileum organ bath preparations was studied during short- (1–5 sec) or long-lasting (20 sec) electrical field stimulation (EFS, 0.8 msec, 40 V, 1–20 Hz). 2. 2. In the presence of phentolamine (5 μM), propranolol (5 μM), and atropine (3 μM), the EFS elicited nonadrenergic, noncholinergic (NANC), tetrodotoxin (0.3 μM)-sensitive responses. 3. 3. The 1-sec EFS evoked relaxation. The response to 5-sec EFS consisted of relaxation followed by twitch, whereas relaxation, twitch and tonic contraction characterized the NANC response to 20-sec EFS. The maximum relaxation was observed at 10-Hz short- or long-lasting EFS. 4. 4. Both N-G-nitro- l-arginine ( l-NNA, 0.1-0.5 mM) and apamin (1–5 μM) concentration dependently inhibited the relaxation of the NANC response to 10-Hz 20-sec EFS. During L-NNA treatment, the twitch and the tonic contractions were increased. The inhibitory effect of L-NNA was reversed by L-arginine (0.1-0.5 mM) but not by D-arginine. Sodium nitroprusside (1–10 μM) was without effect. 5. 5. AP 13.2 ACOH (0.1 μM), a blocker of Substance P receptors, inhibited the twitch and the tonic contractions. The contractions were decreased after desensitization of purinoceptors by ATP and in the presence of the GABA A receptor antagonist bicuculline (30 μM). 6. 6. Depending on the EFS duration, a subsequent occurrence of relaxation and contractions characterized the NANC responses. It seems that relaxation is mediated by nitric oxide whereas Substance P and ATP are involved in the maintenance of the twitch and the tonic contractions. Nitric oxide appears to exert an inhibitory effect on the excitatory transmitters, whereas purinergic mechanism(s) could modulate the nitric oxide-dependent relaxation.