Abstract
The JASMONATE ZIM DOMAIN (JAZ) proteins act as negative regulators in the jasmonic acid (JA) signaling pathways of plants, and these proteins have been reported to play key roles in plant secondary metabolism mediated by JA. In this study, we firstly isolated one JAZ from P. cablin, PatJAZ6, which was characterized and revealed based on multiple alignments and a phylogenic tree analysis. The result of subcellular localization indicated that the PatJAZ6 protein was located in the nucleus of plant protoplasts. The expression level of PatJAZ6 was significantly induced by the methyl jasmonate (MeJA). Furthermore, by means of yeast two-hybrid screening, we identified two transcription factors that interact with the PatJAZ6, the PatMYC2b1 and PatMYC2b2. Virus-induced gene silencing (VIGS) of PatJAZ6 caused a decrease in expression abundance, resulting in a significant increase in the accumulation of patchouli alcohol. Moreover, we overexpressed PatJAZ6 in P. cablin, which down-regulated the patchoulol synthase expression, and then suppressed the biosynthesis of patchouli alcohol. The results demonstrate that PatJAZ6 probably acts as a repressor in the regulation of patchouli alcohol biosynthesis, contributed to a model proposed for the potential JA signaling pathway in P. cablin.
Highlights
Pogostemon cablin (P. cablin), the medicinal part of which is dry whole grass, is a kind of Labiatae plant that has long been considered an important Chinese herbal medicine in Lingnan [1]
We obtained 82,335 raw data of transcriptome using next-generation sequencing (NGS) technology from leaves of P. cablin treated with Methyl jasmonate (MeJA) [18], 12 unigenes were recognized and identified as JASMONATE ZIM DOMAIN (JAZ) family genes, based on the expression levels of 12 genes under the induction of 300 μM MeJA and our previous screening experiment, we chose PatJAZ6 (Unigene48011) as the research object, which may participate in the regulation of biosynthesis of patchouli alcohol
Analysis of the sequence revealed that PatJAZ6 consists of 1375 bp with a 972-bp open reading frame (ORF)
Summary
Pogostemon cablin (P. cablin), the medicinal part of which is dry whole grass, is a kind of Labiatae plant that has long been considered an important Chinese herbal medicine in Lingnan [1]. The pattern of JA signaling regulating the synthesis of secondary metabolites in medicinal plants has progressed in Catharanthus roseus, the main component of which is vinblastine [8]. As a pivotal regulator in the plant JA signaling pathway, JAZ proteins should play important regulatory roles in the biosynthesis of patchouli alcohol in P. cablin. We obtained 82,335 raw data of transcriptome using next-generation sequencing (NGS) technology from leaves of P. cablin treated with MeJA [18], 12 unigenes were recognized and identified as JAZ family genes, based on the expression levels of 12 genes under the induction of 300 μM MeJA and our previous screening experiment, we chose PatJAZ6 (Unigene48011) as the research object, which may participate in the regulation of biosynthesis of patchouli alcohol. The present study is the first to analyze the function of the PatJAZ6 gene in P. cablin, which may be instructive for the study of JA signaling molecular mechanisms and secondary metabolites in P. cablin
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