Abstract
The production of corticosteroids from isotopically labeled cholesterol has been studied in vitro using guinea-pig adrenal slices. 17,21-Dihydroxypregnenolone and 21-hydroxypregnenolone, rather than 17-hydroxyprogesterone and progesterone, were found to be main precursors of cortisol and corticosterone, respectively. The amount of progesterone increased during incubation indicating that only a small part of it, if any, is used for steroid synthesis.
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