Pathway based therapeutic targets identification and development of an interactive database CampyNIBase of Campylobacter jejuni RM1221 through non-redundant protein dataset.

Mohammad Uzzal Hossain,Md Salimullah,Iftekhar Alam,Chaman Ara Keya,Taimur Md Omar,A K M Mohiuddin,Keshob Chandra Das,Patrick Jon Biggs

doi:10.1371/journal.pone.0198170

Mohammad Uzzal Hossain, Md Salimullah + Show 6 more

Open Access

https://doi.org/10.1371/journal.pone.0198170

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Abstract

The bacterial species Campylobacter jejuni RM1221 (CjR) is the primary cause of campylobacteriosis which poses a global threat for human health. Over the years the efficacy of antibiotic treatment is becoming more fruitless due to the development of multiple drug resistant strains. Therefore, identification of new drug targets is a valuable tool for the development of new treatments for affected patients and can be obtained by targeting essential protein(s) of CjR. We conducted this in silico study in order to identify therapeutic targets by subtractive CjR proteome analysis. The most important proteins of the CjR proteome, which includes chokepoint enzymes, plasmid, virulence and antibiotic resistant proteins were annotated and subjected to subtractive analyses to filter out the CjR essential proteins from duplicate or human homologous proteins. Through the subtractive and characterization analysis we have identified 38 eligible therapeutic targets including 1 potential vaccine target. Also, 12 potential targets were found in interactive network, 5 targets to be dealt with FDA approved drugs and one pathway as potential pathway based drug target. In addition, a comprehensive database ‘CampyNIBase’ has also been developed. Besides the results of this study, the database is enriched with other information such as 3D models of the identified targets, experimental structures and Expressed Sequence Tag (EST) sequences. This study, including the database might be exploited for future research and the identification of effective therapeutics against campylobacteriosis. URL: (http://nib.portal.gov.bd/site/page/4516e965-8935-4129-8c3f-df95e754c562#Banner).

Highlights

The genus Campylobacter is composed of a wide variety of non-spore forming Gram-negative bacteria that are predominantly rod or spiral shaped [1]
Most Campylobacter infections are acquired through contaminated food and two species, Campylobacter coli and Campylobacter jejuni are the primary cause of the human disease termed as campylobacteriosis [2,3]
236 chokepoint enzymes were involved in consuming chokepoint reactions and 260 chokepoint enzymes were involved in producing chokepoint reactions

Summary

Introduction

The genus Campylobacter is composed of a wide variety of non-spore forming Gram-negative bacteria that are predominantly rod or spiral shaped [1]. Most Campylobacter infections are acquired through contaminated food and two species, Campylobacter coli and Campylobacter jejuni are the primary cause of the human disease termed as campylobacteriosis [2,3]. Campylobacter jejuni (C. jejuni) is the species that induces acute gastroenteritis and bacterial food poisoning in infected patients. C. jejuni causes the highest proportion of campylobacteriosis cases in developed countries, and in the United States, between $1.3 to $6.8 billion dollars is spent annually for treating the illness [8,9]. Global campylobacteriosis incidence is increasing each year and has almost exceeded the incidence of shigella infections [13,14]. In 2010 New Zealand endured one of the highest rates of campylobacteriosis demonstrating that Campylobacter infection is a global threat [10]

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