Pathway analysis for functional characterization of microRNAs

Abstract

DIANA-miRPath webserver enables the exploration of combined miRNA effects using predicted or experimentally supported miRNA interactions. Its latest version (DIANA-miRPath v4.0, http://www.microrna.gr/miRPathv4 ), introduces the capacity to tailor its target-based miRNA functional analysis engine towards specific biological/experimental contexts. Via a modular interface with rich interaction, annotation and parameterization options, users can perform enrichment analysis on Gene Ontology (GO) terms, KEGG and REACTOME pathways, gene sets from Molecular Signatures Database (MSigDB) and PFAM. Included miRNA interaction sets are derived from state-of-the-art resources of experimentally supported (DIANA-TarBase v8.0, miRTarBase and microCLIP cell-type-specific interactions) or from in silico miRNA-target interactions (DIANA-microT-2023 and TargetScan predictions). Bulk and single-cell expression datasets from The Cancer Genome Atlas (TCGA), the Genotype-Tissue Expression project (GTEx), as well as single-cell expression atlases can be used to assess expression change of targeted genes within terms, across a wide range of states. A discrete module to perform miRNA-tailored CRISPR knock-out screen analyses deems possible the investigation of selected miRNAs within conditions under study. Hellenic Foundation for Research and Innovation (HFRI) under the 1 st Call for HFRI Research Projects to Support Faculty Members & Researchers and Procure High-Value Research Equipment grant (Project Number 2563). This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.