PATHOMORPHOLOGY OF CANDIDAMYCOSIS IN THE LIVER OF REPTILES

Abstract

The article describes our studies on the study of pathomorphological changes in the liver of reptiles with disseminated candidosis. The aim of the research is to study the pathomorphology of mycosis caused by fungi of Candida genus, the features of macroscopic and microscopic changes in liver under the influence of this type of parasitic fungus. The study is aimed at analyzing changes in the hepatobiliary system of reptiles with Candida infection. Pathoanatomical, cytological and histological diagnostic methods were used in the course of the study, with application of: PAS-reaction, Gamorry-Grocott, by Mallory, Van Gieson and Diff-Quikk. Consideration of the issues of pathomorphological manifestation of mycoses, in particular candidosis in the liver of reptiles, was carried out at the Department of Morphology and Expertise of Ural State Agrarian University in 2013 in 2021. To describe the manifestation of yeast-like fungal infections in the liver, we performed a pathoanatomical autopsy of 90 reptiles with a preliminary diagnosis of mycosis, followed by selection, fixation and histological examination of the affected liver according to the generally accepted method. Laboratory research methods were carried out by inoculating biological material on Sabouraud nutrient medium to confirm the diagnosis. We detected changes characteristic of granulomatous process in the liver with candidosis. Granulomas were located both on the surface of the organ and inside. They were pale gray in color, clearly limited, ranging in size from 0.05 to 0.5 cm. The liver parenchyma was loosened, the liver was enlarged, the edges were blunt, the color was from dark red to dark brown. Areas of necrosis with threads of pseudomycelium were revealed in granuloma localization during histological examination of the liver parenchyma. Sinusoidal capillaries were filled with blood in the surrounding tissue, and the vessels in the triad area underwent the processes of mucoid and fibrinoid swelling. Processes of granular, sometimes fatty degeneration were revealed in the parenchyma of the organ.