Pathomorphological changes in experimental model of chronic alcohol and drug-induced pancreatitis in the rat

Abstract

Aim of the research: The aim of the study to conduct the histomorphological study of structural and cellular changes in the tissues of the pancreas, adrenal gland, spleen, liver, kidney in experimental alcoholic and drug-induced pancreatitis. Materials and methods. A model of experimental alcoholic and drug-induced pancreatitis was created on white mongrel rats aged 2-3 months, weighing 25-30 g. The animals were divided into 3 groups (control group No. 1, comparison groups No. 2 and No. 3). A control group (n=10) that did not receive any substances. The second comparison group (n=10) was injected daily with 1 ml of 40% ethanol solution for 10 days. The third comparison group (n=10) was administered intragastric tyrosol daily at the rate of 5 mg per 100 g of body weight for 10 days. Withdrawal from the experiment was performed on the 10 th day from the beginning of the experiment. Laboratory animals were slaughtered by decapitation. Pancreas, spleen and liver, kidneys with adrenal glands were taken for pathomorphological examination. Research results and discussion. During histological examination of the pancreas on the 10th day of the experiment, no changes were noted in the control group, in comparison groups No. 2, No. 3, pathomorphological changes were noted, indicating an inflammatory and dystrophic process in the pancreas. During histological examination of tissues, complications in the liver, spleen, kidneys and adrenal glands were also observed on day 10. Conclusions. The above facts indicate that comparison group No. 2 developed chronic experimental alcoholic pancreatitis induced by ethanol solution; comparison group No. 3 induced experimental drug-induced pancreatitis. One of the main ways of endogenous insufficiency is the enteral pathway, the least dependent on pharmacological correction.