Pathology research of 9L gliomas in Fischer344 rats treated by argon-helium cryosurgery

Abstract

Objective To research the pathological features of 9L/Fischer344 of rat gliomas treated by argon helium cryotherapy.Methods The 9L gliomas were planted into 30 Fischer344 rats to establish the 9L/Fischer344 rat models; these rat models were randomly divided into blank control group (not given any treatment) and argon helium cryotherapy group.Thirty d after that,tumor histopathology was observed; HE staining and electron microscopy were employed to observe the morphology of the tumors; immunohistochemical staining was used to detect the GFAP and S-100 protein expressions.Results In the blank control group,light microscope showed gliomas closely nested arrangement without envelope,minute vessels expanding in the surrounding area of the gliomas,and gliomas growing following the minute vessels; abundant neovascularization,hemorrhage and coagulated necrosis in the gloms were noted; At high magnification,morphological diversity,low differentiated degree,big and excessive nucleus with deep staining were noted in the gliomas; GFAP and S-100 preotein was negatively expressed in the gliomas cell,but positive expression was noted in the glial network between gliomas.In the argon helium cryotherapy group,uniformed coagulation necrosis was noted in the central area,and cell debris and a lot of red and leukocyte infiltration were noted in the marginal zone; a large number of red blood cells,platelets and thrombosis,and visible focal hemorrhage were noted in the capillaries;tissue necrosis or cell apoptosis was noted in all central and marginal zones.Conclusion Argon helium cryotherapy might be an effective way for treating gliomas. Key words: Argon helium cryotherapy; 9L glioma cell; Fischer344 rat; Pathology