Abstract
IntroductionLymph node tuberculosis remains widespread in Cameroon. Our goal was to compare the diagnostic agreement between the hematein-eosin coloration technique and the Ziehl-Neelsen technique.MethodsThis study is a retrospective and comparative study realized in the Yaounde University Teaching Hospital over a period of 5 years. We needed to specify the diagnostic agreement for tuberculosis, first inter observer and secondly between the standard and special colorations. The data we collected allowed us to determine the agreement rates observed and the kappa (k) coefficients with linear weighting.ResultsThe 186 samples of the 1726 cases of tuberculosis of all locations represented a proportion of 10.78%. There were more male patients (65.05%) with a sex ratio (M:F) of 1.30. The average age was 24.21 ± 15.5 with the extremes from 5 to 68 years. The most represented age group was from 10 to 39 years. The two observers agreed in 93 cases using hematein eosin coloration (P0 = 83.87 %; k = 0.8109) and on 73 samples using the Ziehl-Neelsen coloration (P0 = 89.78 %; k = 0.7734). The two coloration techniques presented an agreement on 104 samples (P0 = 88.17 %; k = 0.8783).ConclusionThe routine choice of the hematein eosin coloration technique not paired with the coloration technique of Ziehl-Neelsen can effectively alleviate the program for fighting tuberculosis in an environment of limited resources.
Highlights
IntroductionOur goal was to compare the diagnostic agreement between the hematein-eosin coloration technique and the Ziehl-Neelsen technique
Lymph node tuberculosis remains widespread in Cameroon
The study aimed specifying the diagnostic agreement of tuberculosis in each of the lymph node specimen corresponding to those different subgroups, first inter observer and between standard and special coloration
Summary
Our goal was to compare the diagnostic agreement between the hematein-eosin coloration technique and the Ziehl-Neelsen technique. The two observers agreed in 93 cases using hematein eosin coloration (P0 = 83.87 %; k = 0.8109) and on 73 samples using the Ziehl-Neelsen coloration (P0 = 89.78 %; k = 0.7734). In Cameroon the national program for the fight against tuberculosis (PNLT) counted 26 110 cases of TB, all forms taken together, of which 15 080 were smear positive cases This corresponds to a notification rate of 124 and 73 for 100 000 inhabitants respectively [3]. The interest of the present study whose principal goal was to compare the diagnostic agreement between the standard coloration technique with the special Ziehl-Neelsen technique
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