Pathology and Epidemiology of Fungal Infections in Layer Chicken Flocks

Abstract

Fungal infections have a key effect on the commercial poultry production and welfare. Infections caused by fungi and their food contaminants are zoonotic and influence food safety. Eggs and cooked meats remain major public health concerns. Therefore, this research is aimed at examining the pathology and understanding the epidemiology of fungal infection in layer chicken flocks. The study was carried out on twenty-layer flocks from Kafrelsheikh Governorate, Egypt, from January 2019 to December 2020. In total, 600 samples were collected from 100 healthy and diseased layer chickens from different organs (skin, liver, lung, kidney, spleen, and ovary). In this work, we present the clinical and pathological characteristics of some fungal pathogens (Aspergillus spp. and Fusarium spp.) in layer chicken flocks, as they are responsible for reducing the egg production. In total, 19 fungal strains were isolated from individual chickens, and these were analysed to determine the fungal species. The total proportion of fungal infections at the farm level was (3/20) 15%. The main clinical signs were emaciation and mortalities that reached 4.34 % ± 0.84 . We report the first isolation of Aspergillus piperis and Fusarium species from the ovary of poultry, which is the main reason for egg retention and multiple numerous nodules of occasional caseating centers in layer ovaries. The histopathological findings of Aspergillus infection are indicated by the presence of branched hyphae that tend to be numerous and progressive. Furthermore, we found spherules with multiple endospores of Fusarium spp. in the ovaries. Morphological and molecular identification and analysis were performed to confirm the etiological agents.