Abstract
Objective To observe the pathological change of the co-transplantation of neural stem cells(NSC),Schwann cells(SOs)and PLGA to the spinal cord injury in rats.Method Thirty-six Wistar rats were divided into three groups randomly:PLGA,NSC/PLGA and NSC+SCs/PLGA groups.The complex of PLGA-cells was constructed and implanted into the injury site of T9 SCI models developed from Wistar rats.All the SCI tissues were observed with electromicroscope,HE and immunohistochemistry to identify the bioeompatibility of PLGA,regeneration of neural fibers and the survival,migration and differentiation of the transplanted cells at 4.8 and 12 weeks after injury.Results Different cells and regenerated blood capillary were detected in the scaffolds through HE stain.The PLGA gradually degenerated under scanning electromicroscope.The regenerated axons were observed in the scaffold-cells complex with electromicroscope.Immunohistochemistry staining showed the transplanted NSC could survive and migrate at 12 weeks and differentiated into neurons and oligodendrocytes.Conclusions Co-transplantation of NSC +SCs/PLGA can morphologically promote the reparation of the injured spinal cord. Key words: Spinal cord injury; Neural stem cells; Schwann cells; PLGA; Pathology
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