Abstract
BackgroundThis study is to explore the pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.MethodsCD133 positive TJ905 glioma cells were separated by immunomagnetic beads to isolate glioma stem-like cells. TJ905 cells and stem-like cells were inoculated subcutaneously into the mice to establish model of transplanted tumor, respectively. Mice growing condition and behavior were observed. HE staining assay, immunohistochemical assay for GFAP, Ki-67 and Olig-2, and CD34 marked microvascular density (MVD) test were performed.ResultsThe growing condition and behavior of mice in TJ905 stem cell group was more exaggerated and the models showed stronger malignant features pathologically than that in TJ905 cell group. Glial fibrillary acidic protein (GFAP) in TJ905 cell and stem-like cell group showed the transplanted tumor originated from astrocytes. Expression of Ki-67 and oligodendrocyte transcription factor-2 (Olig-2) in TJ905 stem cells was higher notably and CD34 expression in stem cell group was significantly higher than that in the other two groups.ConclusionsPathological features of transplanted tumor established by CD133 positive glioblastoma stem-like cells show more malignant. Use of TJ905 stem cells to establish transplanted tumor model in nude mice is excellent for glioma research.
Highlights
Glioblastoma Multiforme (GBM) is the most common adult primary malignant brain tumors
Standard surgical resection with radiotherapy and chemotherapy, is performed, the median survival time of patients is 12 to 18 months and most patients died within 2 years after diagnosis [2]
Current evidence indicates that a subpopulation of cancer cells, named cancer stem cells (CSCs) or tumorinitiating cells which are responsible for the initiation, growth, metastasis, therapy resistance and recurrence of cancers, share core regulatory pathways with normal stem cells but rely on distinct reprogrammed pathways to maintain stemness and to contribute to the progression of cancers [4]
Summary
Glioblastoma Multiforme (GBM) is the most common adult primary malignant brain tumors. Many studies found that there are a small group of cancer stem-like cells in glioblastoma multiforme and they have features similar to normal neural stem cells, such as self-renewal and differentiation capacity. The group of cells might be an important cause for recurrence of GBM and resistance to chemotherapy [5]. Those glioma stem cells show characteristics including higher tumorigenic potential, Jin et al Cancer Cell International (2015) 15:60 low multiplication rate, high expression of CD133 and specific genes of neural stem cell [6]. This study is to explore the pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells
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