Pathological changes inducing by Brucella mellitensis in mice immunized with Culture Filtrate Brucella mellitensis Antigens (SCFAgs) and chitosan

Abstract

In order to determine the influence of Culture Filtrate Brucella mellitensis Antigens (CFAgs) on B. mellitensis infection in chatosan immunostimulater mice, sixty for white mice, both sex,7-8 weeks age were divided randomly into for groups.1st group(n=16 ) was immunized with 0.4ml of CFSAgs B. mellitensis (concentration of protein( 4.2mg/ml) ,i/p two doses, 2 weeks intervals. 2nd group(n=16) was feed on diet supplement with chitosan ,(1mg/kg B.W) 4 weeks. group. 3ed group(n=1) was inoculated with (0.4ml) I/P with1X109 CFU/ML of viable virulent B. mellitensis and was served as control positive group. 4th group (n=16) was inoculated with 0.5ml sterile normal saline. Cellular and humoral immune response were recorded at 28-30 day post immunization, skin test and passive heam agglutination test respectively, then all animals of immunized and feed chatosan groups were challenge with B. mellitensis as control positive group. The results explained that dead for animals in cotral positive during 15 days post inoculation with virulent viable B. mellitensi with very heavy bacterial isolation, from animal of control positive group post infection The results revealed that immunization with CFSAgs elicited both humoral and cellular immune responses, the level values of both arms of immune response also result reveald that immunization with CFSAgs + chatosan elicited both humoral and cellular immune responses higher than other group , Severe pathological lesions were seen in examined organs of control positive group but these lesions are mild or few in animal immunization with CFSAgs + chatosan. The main lesions in examined organs of these animals are suppurative inflammation ,small grnulomma .. We conclusion that immunization with CFSAgs + chatosan can improve the immune responses in the animals that are suffering from Brucella mellitensis infection