Abstract
A Total 135, 1-3 months aged rabbits were used for this study. Faecal swabs were aseptically collected from different rabbit farms in Assiut Governorate. Diarrhea, ruffled fur and depression were observed in 45 out of them, while the rest were apparently healthy and 8 rabbits were chosen to be the control. These samples were examined bacteriologically for determination of the occurrence and frequency ofStaph. aureus. Thirty three isolates from the diseased rabbits at percentage of (24.4%) were positive for Staph. aureus. Experimental infection in 6-8 week-old rabbits via subcutaneous (S/C) injection of isolated Staph. aureus led to 75% mortality with post-mortem lesions similar to a great extent to those of natural infection. Reisolation of infecting organism from internal organs and intestinal tract of dead and slaughtered rabbits at the end of observation period were conducted. Histopathological examination of the affected tissue revealed signs of septicemia and suppuration in various organs.
Highlights
أجرٌت هذه الدراسة علً عدد 135من الأرانب التً تتراوح أعمارها بٌن شهر إلى ثلاث شهور لدراسة مدى تعرض مزارع الأرانب الخاصة بمحافظة أسٌوط للإصابة بالمٌكروب المكور
Round golden-yellow colonies, with beta-haemolysis of blood agar (Fig 1a,b).According to morphological and bio-chemical characters, 33 isolates (24.40%) were identified to be Staph. aureus (Table 1 and Fig 2). These isolates were Gram positive cocci usually arranged in clusters and characterized by its typical growth of coagulase positive, black colonies surrounded by faint yellow zone on Baird-Parker agar media and its haemolytic activity on blood agar media
2- Experimental infection in rabbits Results of pathogenicity tract are given in Table (2) and Fig. (3)
Summary
1- Collection of samples: Fecal swabs were collected aseptically from 135 rabbits from different farms in Assiut Governorate for investigating the occurrence and pathogenicity of the Staph. aureus. 1- Collection of samples: Fecal swabs were collected aseptically from 135 rabbits from different farms in Assiut Governorate for investigating the occurrence and pathogenicity of the Staph. 2- Bacteriological examination: a- Samples: Tissue samples from liver, heart, lungs, kidneys, as well as samples from suppurative lesions were collected and subjected to bacteriological examination. Strains: Samples from individual rabbits including liver, lungs, kidneys, and pus were collected aseptically. Lopopfull from these samples were inoculated into sodium chloride broth and incubated at 37 oC for 18-24 hour, followed by sub-culturing on solid media as: Baried-parker agar media at 37 oC for 48 h as a selective medium for staphylococci (Bairdparker, 1962), Blood agar and nutrient agar media at 37co C for 24 h. Serial sections of 4μm thickness were obtained and stained with hematoxylin and eosin (H and E) according to Bancroft et al (1996)
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