Pathologic characteristics and cell cycle regulatory protein expression as a prognosis marker after curative resection in gall bladder cancer

Abstract

15634 Background: We investigated pathologic characteristics, ERCCI, survivin, Thymidine phosphorylase (TP) and some cell cycle regulatory proteins to determine the prognostic and predictive value in gall bladder cancer after curative surgery. Methods: We reviewed the pathologic parameters, including tumor tissue differentiation, lymphatic, perineural, and venous invasion in formalin-fixed, paraffin-embedded block from 73 gall bladder cancer tissues after curative surgery. We also performed immunohistochemical staining for ERCC1, survivin, TP, p53, cyclin D1 and bcl-2 to determine the association with clinical course. Results: Male and was 33 and 40 cases(45.2% vs. 54.8%), respectively and the median age was 62 year old. Of the 73 cases, TP expression showed in 54.8%, p53 in 43.8%, survivin in 27.4%, ERCC1 in 24.7% and cyclin D1 in 23.3%. In correlated with prognosis and pathological parameters, lymphatic invasion had statistically significant association with recurrence (p=0.033), showing less in negative group. Histologically, the group of well differentiated tumor had better survival outcome than those of poor differentiated tumor (p<.001), and positive perineural invasion showed poorer survival than negative group (p=.027). However, no significant correlation was found between survivin, ERCC1, thymidine phosphorylase (TP), p53, cyclin D1 and bcl-2 expression and survival or recurrence. Conclusions: In gall bladder cancer after surgery, pathologic characteristics, such as lymphatic, perineural invasion or tissue differentiation can be a useful prognostic marker for survival or recurrence. No significant financial relationships to disclose.