Pathologic changes of pancreatic endothelial cells in Diabetic Rat

Abstract

Aim: The study is to explore the relationship between pancreas endothelial cell injury and diabetes. Methods: To induce with a single intraperitoneal injection of streptozotocin (STZ) for diabetic animal models, and collected the blood after 8 weeks, the level of Angiogenin-1 (Ang-1) and its receptor (Tie-2), vascular cell adhesion molecule-1 (VCAM-1) were determinated by ELISA method, histopathological examination of the pancreas, and observed pathological conditions of the pancreas endothelial cell injury in the models. Results: The results showed that the level of ang-1, tie-2 and VCAM-1 were 585.04 ± 45.32 (pg/ml), 2399.40 ± 124.85 (pg/ml), 117.69 ± 12.34 (μg/L) in the Diabetes groups which were higher than that in control groups 551.29 ± 31.86 (pg/ml), 2103.27 ± 152.89 (pg/ml) and 93.43 ± 10.48 (μg/L), (t=3.78, 3.52, 7.52, P<0.05). Conclusion: The Vascular endothelial cell factors of e NOS, VCAM-1, SMA, VEGFR and ICAM-1 were higher expressed in Diabetes mellitus rats than that in the normal control groups. We found that there is a certain correlation between the development of pancreatic endothelial cells and the development of diabetes, and the treatment of diabetes can provide some ideas for the future.