Abstract
Maesopsiseminii Engl. is a commercial wood which classified as fast-growing multifunctional species. The disease will inhibit plant growth as well as decrease the quality of timber. Botryodiplodiatheobromaeis disease-producing pathogen which is harmful and has many hosts. This study aims to examine the pathogenicity of Botryodiplodiatheobromae, which harms the plant seedling leaves both macroscopically and microscopically. The experimental used a completely randomized design. The treatments consisted of inoculation without wounding, wounding, wounding with inoculation. The results showed that there was a symptom of brownish-colored spot occurred on plant seedling leaves. According to the Duncan’smultiple range test (DMRT), wounding with inoculation provided the greatest average value of disease severity by 27.56% and disease incidence by 82.67%. The results of macroscopic observation on PDA showed that colony of isolates until seven days old had white-colored mycelium, while the old mycelium looked grayish to black. Mycelium colony had a texture which is similar to the fine threads with thick aerial mycelium and dispersed unevenly from the middle part of mycelium. Microscopic observation on the symptomatic leaf disease pathogens result showed Botryodiplodiatheobromae characteristics, such as sectional hyphae and ellipsoidal conidia hyaline.
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