Pathogenicity and vegetative compatibility among isolates of Fusarium oxysporum and F. moniliforme colonizing asparagus tissues

Abstract

Isolates of Fusarium moniliforme (Sheld.) emend. Snyd. & Hans., F. oxysporum (Schlecht) emend. Snyd. & Hans., and F. solani (Mart.) Appel & Wollenw. emend. Snyd. & Hans. were recovered from three 5-year-old field grown asparagus (Asparagus officinalis L. cv. Mary Washington) by isolating from symptomatic and asymptomatic feeder roots, storage roots, crown and basal stem segments. Fusarium moniliforme was more virulent than F. oxysporum on asparagus seedlings and F. solani was considered nonpathogenic. Isolates of F. moniliforme and F. oxysporum were placed into vegetative compatibility groups (VCGs) by demonstrating heterokaryosis with complementation tests using nitrate-nonutilizing (nit) mutants (pairing nitM and nit1 mutants). Ninety-seven of 135 isolates of F. moniliforme were placed in 13 vegetative compatibility groups. The remaining 38 isolates were not classified by vegetative compatibility because of poor nit mutant recovery. Eight of 18 isolates of F. oxysporum were unique and classed as single members of eight different VCGs. The other 10 isolates were not placed in VCGs. All isolates of F. moniliforme were virulent, but mean disease ratings differed among the isolates in different VCGs. There was no correlation between vegetative compatibility group and tissue substrate or symptom expression on the tissue substrate. It appears that virulence on asparagus is a common trait with few exceptions among genetically distinct populations of F. moniliforme and F. oxysporum colonizing asparagus.