Pathogenic characteristics of the Vibrio parahaemolyticus which caused a gastroenteritis outbreak event in Huzhou.

Abstract

The pathogenic characteristics of V. parahaemolyticus isolated from a gastroenteritis outbreak event in Deqing County of Huzhou City in 2022 were analyzed. Pathogen detection was performed on 30 anal swabs (26 patients, 1 chef and 3 waiters). The isolates of V. parahaemolyticus were analyzed by serum typing, pulsed field gel electrophoresis (PFGE) molecular typing, multiplex fluorescent PCR detection of tdh/trh virulence gene and drug sensitivity test. 15 patients were positive for V. parahaemolyticus, 1 patient was positive for V. parahaemolyticus and Enteroaggregative E. coli (EAEC), 1 patient was positive for EAEC, and the chef was positive for EAEC. The serotype test results of the 16 V. parahaemolyticus were 14 O4:KUT and 2 O10:K4. All samples were negative for other tested bacteria. All V. parahaemolyticus strains were positive for tdh genes and negative for trh gene. The 16 isolates were 100% resistant to ampicillin (AMP), and sensitive to the other12 antibiotics. From the results of serotype and PFGE, the V. parahaemolyticus strains with two serotypes are clustered into two branches according to their serotypes. The three EAEC strains were non-homologous. In conclusion, we detected V. parahaemolyticus and EAEC from an outbreak of gastroenteritis. And V. parahaemolyticus with two serotypes may be the cause of this event, according to the traceability results.