Pathogenic and morphological variability among the isolates of Fusarium udum (Butler) causing wilt of pigeonpea (Cajanus cajan)

Abstract

Wilt is the most serious disease causing irreversible losses and lethal damage to the crop in Bihar, Jharkhand, Orissa and the West Bengal states of Eastern India. Because of the existence of high pathogen variability, management of the disease using resistant cultivars will be a challenging task. For breeding resistant varieties, knowledge of pathogen variability in the particular crop area is essential. In present study, fifteen isolates viz., Fu-9, Fu-10, Fu-27,Fu-32, Fu-42, Fu-49, Fu-63, Fu-72, Fu-73, Fu-74,Fu-75,Fu-84, Fu-86, Fu-87, Fu-97of Fusarium udum obtained from wilt infected pigeonpea plants of Bihar, Jharkhand, Orissa and West Bengal of Eastern India were assessed for the phenotypic variation. The isolates exhibited considerable variations in cultural and morphological characters viz., radial growth, mycelial color, sporulation, size and shape of macroconidia. Pathogenic variability on soil inoculated pot grown plants of pigeonpea resulted in 12.0 to 57.1 percent wilt incidence. The isolates also exhibited significant variations in symptoms like drooping of shoots, latent period, timing of fungal invasion, vascular clogging, drooping of shoots, and wilt establishment etc. Based on the wilt incidence, the fifteen isolates were distinguished into pathogenic groups. Fu- 10, Fu-27, Fu-32 and Fu-49 were found highly pathogenic and predominantly causing the typical wilt symptoms of more than one 31% wilt incidence. The present study indicates the existence of variability among isolates of vascular wilt pathogen collected from Eastern region of India.