Abstract
The purpose of this study was to study the cotransplantation influence of multipotent mesenchymal stromal (MMSC) and hepatic stellate (HSC) cells on liver regeneration of old laboratory animals in conditions of its toxic damage. Acute toxic hepatitis was caused by single intraperitoneal CC14 injection at a dose of 50 μg/kg. The introduction of MMSC and HSC was carried out at doses of 4 million cl/kg and 9 million cl/kg respectively 1 hour after toxic hepatitis modelling. The morphofunctional liver state of old laboratory mice was evaluated on the 1st, 3rd, 7th day after combined injection of MMSC and HSC in laboratory animals with toxic hepatitis. As a result of the study, it was obtained that MMSC and HSC cotransplantation leads to cellular and intracellular liver regeneration activation in old mice with acute toxic hepatitis. Also, the introduction of these cell types leads to decreased liver mutagenesis, inhibition of programmed cellular hepatocytes death. Thus, the conducted studies indicate the ability of combined MMSC and HSC transplantation to restore the morphofunctional liver state of the old organism under the conditions of its toxic damage.
Highlights
Statistics data analysis from the Ministry of Health of the Russian Federation, the Department of Monitoring, Analysis and Strategic Development of Health, as well as the World Health Organization for 2018 show an increase in the number of patients with liver pathology [1]
This study examined the ability of two types of stem cells — hepatic stellate cells (HSC) and multipotent mesenchymal stromal cells (MMSC) to activate liver regeneration in conditions of its toxic damage through the carbon tetrachloride introduction
Conducted studies indicate the ability of combined MMSC and HSC transplantation to activate liver regeneration in the old body under the conditions of its toxic damage
Summary
This study examined the ability of two types of stem cells — hepatic stellate cells (HSC) and multipotent mesenchymal stromal cells (MMSC) to activate liver regeneration in conditions of its toxic damage through the carbon tetrachloride introduction. The choice of these types of stem cells was due to their biological properties. The ability of MMSC through formation of intercellular contacts to induce the production of heat-shock proteins with molecular weight of 70 kD (chaperones) in cells [9, 10, 11] has been proved This group of proteins supports the conformation of structural and functional proteins. Given these biological relationships between MMSC and HSC, it seems promising to study the effect of combined transplantation of these cell types on liver regeneration in conditions of its damage
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