Pathogenetic features of formation of experimental preeclampsia-like condition

Abstract

Objective. To study the pathogenesis of clinical and laboratory syndromes of preeclampsia-like condition and the possibility of influencing them through the introduction of specific antibodies to marinobufagenin.
 Materials and methods. 36 pregnant rats were used in the experiment. 24 rats from 12 to 19 days of pregnancy were prescribed a 1,8% NaCl solution to simulate a preeclampsia-like condition. Subsequently, this sample was divided into 2 groups. In one group (12 animals, the second group), preimmune rabbit serum was administered intraperitoneally as an immune agent. In another group (12 animals, the third group), a single intraperitoneal administration of polyclonal anti-marinobufagenin antibodies was performed. After the antibody injection, a comprehensive clinical and laboratory evaluation was performed.
 Results. Against the background of a hypersol diet, an increase in systolic blood pressure was found, as well as the concentration of marinobufagenin in blood plasma. The introduction of antibodies to marinobufagenin caused a decrease in blood pressure. In addition, the formation of a preeclampsia-like condition showed an increase in endoglin and sFlt1 in the placenta, as well as an increase in sFlt1, collagen-1 and a decrease in Fli-1 in the thoracic aorta. The appointment of antibodies to marinobufagenin led to an increase in the content of Fli-1 to the level in the control group.
 Summary. In the formation of a preeclampsia-like condition, an increase in the level of marinobufagenin in blood plasma leads to an increase in the content of antiangiogenic factors in the placenta and in the thoracic aorta. Intraperitoneal administration of antibodies to marinobufagenin contributes to a significant increase in the content of Fli-1.