Pathogenesis-related proteins in barley leaves, induced by infection with Drechslera teres(Sacc.) Shoem. and by treatment with other biotic agents

Abstract

The perthotrophic fungus Drechslera teres, the causal agent of net blotch disease in barley, induces the accumulation of pathogenesis-related (PR) proteins in barley leaves as shown by isoelectric focusing. The same protein pattern was also found in leaves treated with a toxin extract from the culture filtrate of D. teresas well as after infection with Erysiphe graminisf.sp. hordeior Puccinia hordei. Some of the proteins induced by infection with D. tereswere characterized as peroxidases, β-1,3-glucanases and chitinases by isoenzyme analysis. Immunodetection following western blots demonstrated that the induced proteins are the same as those that accumulate after inoculation of barley with E. graminis: basic PR-1a and b proteins, thaumatin-like (TL) proteins, β-1,3-glucanases and chitinases. The accumulation of PR-1 type proteins, chitinases and TL-proteins was analysed quantitatively by ELISA.