Pathogen detection and its clinical characteristics of children with ordinary hand, foot and mouth disease

Abstract

Objective To investigate the characteristics of general clinic data of children with ordinary hand, foot and mouth disease(HFMD) and the serotype distribution of enterovirus(EV) detection results, and to explore the epidemiological characteristics of ordinary HFMD. Methods From March to September, 2014, a total of 193 children who were diagnosed as ordinary HFMD in department of clinic service, Chengdu Women and children's Central Hospital, were chosen as study objects.The throat swab samples from the 103 children with their parents'permission, were collected and analyzed by real-time fluorescence polymerase chain reaction(RT-PCR) to get the EV serotypes. Clinical characteristics of 193 children with ordinary HFMD were summarized and compared the clinical characteristic differences among infection of Coxsackie virus(Cox)A16, EV71 and other EV by statistical method.The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Chengdu Women and children's Central Hospital.Informed consent was obtained from the parents of each participant. Results ①Among 193 children with ordinary HFMD, 118 cases(61.1%) were 1-3 years old, 167 cases(86.5%) were 1-5 years old; 119 cases(61.7%)were scattered children.Oral mucosal pox or ulcer as well as hand, foot and buttocks rashes were observed in all 193 children, and the prognosis were good for all subjects.②The EV positive detection rate of throat swab samples of 103 ordinary HFMD children was 86.4%(89/103). Among the 89 ordinary HFMD children with EV positive, 57 cases(18.0%)were CoxA16 positive, 16 cases(18.0%) were EV71 positive, and 16 cases(18.0%) left were other EV positive.③Among the 89 ordinary HFMD children with EV positive, there were no significant differences among infection of CoxA16, EV71 and other EV in general clinic data such as the age, constituent ratio of gender and living range, positive rate of allergy history, etc.; Or in the clinical manifestations of fever rate, peak temperature, fever lasting time and incidence of complications; Also in laboratory examination such as white blood cell count and C-reactive protein(CRP), etc.(P>0.05). Conclusions CoxA16 infection was the main pathogen of ordinary HFMD in Chengdu Women and children's Central Hospital in 2014. The characteristics of clinical manifestations, white blood cell count and CRP content results of ordinary HFMD children can not speculated the possible serotype of EV. Key words: Hand, foot and mouth disease; Coxsackievirus infections; Enterovirus A, human; Child