Abstract
The inheritance of chloroplast DNA in Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) was determined using a restriction fragment length polymorphism (RFLP) as a marker. An insertion/deletion sequence homologous to a 15.3 kilobase PstI fragment from petunia chloroplast DNA was detected in SmaI and BamHI digests of total DNAs of parent trees. Full-sib progeny (36 in total) were then assayed for this polymorphism in three sets of crosses between male and female parents with different RFLP markers. The full-sib progeny had the same restriction fragment as the male parent, with three exceptions. These data provide direct evidence for the paternal inheritance of chloroplast DNA in a gymnosperm. This result is in sharp contrast to the strict maternal and occasional biparental inheritance of chloroplast DNA observed in angiosperms. The three exceptions had restriction fragments unlike either the male or female parents of the cross, suggesting that some type of mutational or recombinational event had occurred during the transmission of these genomes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
